Confocal microscopy enables to overcome the diffraction limit of lateral resolution inherent to conventional light-optical microscopy and can deliver blur-free images of thick specimens, suitable for 3D reconstruction.
The confocal microscope is a tool allowing researchers to examine cells, tissues and samples in a specific way and thus opening the door to new investigation and collaborations. The microscope has a shallow depth field so an image is collected from an optical diction rather than from the full depth of the specimen as in conventional light microscopy.
Applications
The 3D-imaging properties of the CLSM make it an ideal tool to study cellular topography, internal structure, and fluorescence properties of biological objects and materials with dimensions in the sub-mm to mm range.